Nicole Vo PhD

Medicine/Nephrology
Acting Assistant Professor

Email: nicolevo@uw.edu | Phone: 857.337.5014

I am developing advanced methods to achieve efficient and precise gene editing in a variety of human organoids using CRISPR-Cas9 and base editing technologies. This work includes a platform for gene knock-in and knock-out utilizing Cas9 and gRNA ribonucleoprotein (RNP) complexes, as well as targeted nucleotide modifications through base editing machinery. To monitor gene editing events across various cell types, I am implementing a novel fluorescence-on reporter system in human induced pluripotent stem cells and derived organoids. Additionally, I am working to enhance targeting specificity and efficiency by tethering Cas9 to molecular targeting agents (MTAs), enabling precise editing in desired cell populations. These innovations provide versatile tools for gene therapy development, offering scalable and off-the-shelf solutions for addressing diverse disease states.