Sudarshan Pinglay PhD
UW Genome Sciences
Research Assistant Professor
Email: pinglay@uw.edu | Phone:
Our team develops technologies that bring together mammalian genome engineering, single cell sequencing and de novo biological design. We seek to understand how gene expression is controlled as cells transition between different states, to examine how alterations in genome structure - called structural variants (SVs) - can contribute to disease and ultimately aim to engineer cells with sophisticated capabilities for cell therapy and regenerative medicine.
To tackle these challenges, we aim to engineer stem cells with defined SVs to model their clinical impact. This enables us to study how genome alterations influence development and function at an unprecedented scale. In parallel, we are developing methods to deliver large synthetic DNA sequences - over 100,000 bases long - into mammalian cells. These large constructs allow us to precisely edit complex genomic regions and program stem cells with new functions, such as guiding their differentiation or recording their developmental history. The goal of our work is to create new tools for studying and treating disease, with long-term benefits for genomic variant interpretation in the clinic, biologics production, cell therapy, and regenerative medicine.