With a long-term goal of improving dental health outcomes, ISCRM Fellow Anjali Patni will generate functional enamel layer in order to study the congenital tooth disorder amelogenesis imperfecta (AI) and to generate living fillings in the future. In her project, Patni aims to identify the signaling factors crucial for the maturation of induced secretory ameloblasts (isAM) and investigate the underlying mechanisms of Amelogenesis Imperfecta (AI) using an iAM 3D organoid model. As part of her investigation, Patni will utilize previously AI-designed Delta scaffolds to activate the Notch pathway in target cells. By utilizing these innovative scaffolds, Patni will explore whether Notch pathway activation alone is sufficient to induce the maturation of ieAM into isAM. Through these comprehensive efforts, Patni’s research holds potential to advance our understanding of amelogenesis imperfecta and contribute to the development of innovative strategies for dental health improvement.