With funding from the ISCRM Undergraduate Fellowship, Sahana Subramanian will identify healthy and unhealthy endothelial cell flow conditions within a perfusable engineered heart tissue, hypothesizing that physiologically relevant shear stress will lead to endothelial cell alignment and strong barrier properties while predicting that low shear stress will lead to signs of endothelial dysfunction, such as permeable barriers and inflammatory signaling. The goal of this project is to interrogate endothelial function under several hemodynamic conditions in this perfusable model.