Cystic Fibrosis (CF) is a life-limiting genetic disease caused by a mutation of CFTR, eventually leading to respiratory failure. With an ISCRM fellowship award, Jasime Villegas aims to create a CRISPR-Cas9 CF mutant iPSC-derived lung organoid model that reflects the pathophysiological responses of CF, such as reduced mucociliary clearance and respiratory infection responses. These LOs exhibit important cellular phenotypes, such as mucins and goblet cells, that are essential to understanding these responses. Success of this project will create novel platforms for investigating cellular mechanisms and developing CF therapeutics.